哈尔滨医科大学八十周年校庆系列学术活动

哈尔滨医科大学八十周年校庆系列学术活动 ——如何进行科学实验研究的基础训练课

5月30日

9:30-11:30AM 如何进行科学研究(概述)和如何复习文献 （唐朝枢教授） 2:00-4:00PM 代谢综合征（核转录因子及实验模型应用） （管又飞教授） 4:00-6:00PM 转录因子调控相关实验研究 7:30-9:30PM 组织特异基因敲除技术及其应用 5月31日

8:00-10:00AM 如何设计研究课题 10:00-12:00AM 蛋白质组学技术及应用 2:00-4:00PM 如何撰写实验研究论文 4:00-6:00PM 如何撰写和发表英文论文 7:30-9:30PM 如何作科学学术报告 6月1日

8:00-12:00AM Western blot技术详解:问题与对策 2:00-4:00PM 病毒载体技术及疾病的基因治疗 4:00-6:00PM RNA沉默及microRNA技术 地点：主楼后楼科技会馆

北京大学医学部生理和病理生理系(负责教师：系主任 王宪教授) 教育部和卫生部心血管分子生物学重点实验室 教育部创新团队

（朱毅教授） （管又飞教授）

（唐朝枢教授） （王宪教授） （唐朝枢教授） （王宪教授） （朱毅教授）

（徐国恒教授） （汪南平教授） （汪南平教授）唐朝枢 教授 简介

唐朝枢，男，教授，博士生导师。1968 北京医学院医学系毕业，1981获北京医学院病理生理学专业硕士学位。1990年晋升为教授。1993年评为博士生导师。曾四次赴美国进修和合作研究。曾任国家九七三项目（心脑血管疾病发病和防治基础研究）首席科学家，长期从事心血管生理及病理机制方面的科研和教学工作。兼任《生理学报》、《生理科学进展》、《北京大学学报（医学版）》，《中国动脉硬化杂志》，《中华老年多器官功能障碍杂志》和《国际病理科学和临床杂志》副主编，《中华医学杂志》、《中华心血管病杂志》和《中国医学科学院学报》等杂志编委。参编、主编《病理生理学》教科书及《休克学》、《老年心脏病学》和《动脉粥样硬化性心血管病.基础与临床》等专著多部。至今已发表SCI收录论文壹百余篇。曾获国家科技进步二等奖（1998年），部委一等奖（1991、1997、2003和2005年）、二、三等奖等多项奖项。

82802183. E-mail: tangchaoshu@263.net.cn

王 宪 教授 简介

王 宪 教授, 博士生导师. 1980.1北京医学院医疗系毕业, 1985年获病理生理学硕士学位, 1992年获生理学博士学位. 曾在美国芝加哥劳约拉大学医学院生理系和免疫系以及美国肯塔基州立大学医学院生理系进修4年多.曾获首届国家杰出青年科学基金, 第二届全国中青年医学科技之星; 中国青年科学家奖;香港求是科技基金会设立的\杰出青年学者奖\；2000年被聘为教育部长江特聘教授.现任北京大学基础医学院生理学与病理生理学系主任. 兼任中国生理学会常务理事，【生理科学进展】常务编委等。十几年以来，共独立主持包括美国洛克菲勒生物技术专业基金、国家杰出青年科学基金、美国中华医学血管生物学基金子项目、心血管国家重点基础研究发展规划\子项目和国家自然科学基金重点项目等十几项科研课题的研究工作。她的实验室主要从事神经-免疫调节机制及其在心血管疾病发病中意义的研究工作,特别是关于动脉粥样硬化发病时血管炎症免疫的发病机制和抗炎症保护机制.该室研究特点是采取跨学科研究，将免疫学与心血管疾病发病机制研究紧密结合，将基础医学与临床医学的研究紧密结合.此外，近年来还采用蛋白质组学技术,寻找参与心血管缺氧/预适应和动脉粥样硬化发病的新的内源性介导物质. 王宪已发表原著论文120余篇，其中在国际SCI杂志上发表的文章60余篇。代表性5篇研究论文如下:

1．Wang, W., L. Jia, T. Wang, W. Sun, S. Wu, X. Wang*. Endogenous calcitonine gene-related peptide protects human alveolar cells through protein kinase C? and heat shock protein. J Biol Chem, 2005, 280:20325-20330．

2．Li, W, L. Hou, Z. Hua, X. Wang*. Interleukin-1β induces β-calcitonin gene-related peptide secretion in human type II alveolar epithelial cells. FASEB J, 2004;18:1603-1605

3． Zeng, X., D. Remick, X. Wang*. Homocysteine induces secretion and expression of chemokine MCP-1 and IL-8 in human monocytes. Circ.Res.2003, 93:311-320

4．Zhang, Q., J. Guo, X. Wang*.. Oxidant stress mechanism of homocysteine potentiating Con A-induced proliferation in murine splenic T lymphocytes. Cardiovas. Res. 2002, 53:1035-1042.

5．Xing, L.,J. Guo, X. Wang*. Induction and expression of beta-calcitonin gene-related peptide (b-CGRP) in lymphocytes and its significance. J. Immunol. 2000, 165：4359－4366.

徐国恒 教授 简介

徐国恒，男，1964年生，医学博士；北京大学医学部生理学系教授，博士研究生导师；教育部分子心血管重点实验室Principle Investigator。1987年河南医科大学临床医疗系本科毕业获学士学位; 1990年于河南医科大学药理学系获医学硕士学位，1995年于中山医科大学药理系获医学博士学位。1995-1997年北京医科大学神经科学中心做博士后。1997年底赴美国国立卫生研究院(NIH)儿童健康与人类发育研究所(NICHD)内分泌生理实验室做博士后研究员，研究促肾上腺皮质激素释放激素受体基因调控。1999年底转入美国NIH糖尿病消化和肾病研究所(NIDDK)细胞发育生物学实验室做博士后研究员，研究细胞内脂滴(Lipid Droplet)及脂滴包被蛋白的细胞生物学，以及脂肪分解代谢、肥胖、糖尿病胰岛素抵抗的分子机制。2002年底回国于北京大学医学部生理系工作。目前承担2项国家自然科学基金面上项目，1项北京市自然科学基金面上项目，1项新世纪优秀人才支持计划基金。2004年入选教育部新世纪优秀人才支持计划。任《生物学通报》常务编委、《中华糖尿病杂志》特约编委。 研究方向：

1. 脂肪分解和脂肪酸运动的分子调控机制

2. 肥胖、胰岛素抵抗、泡沫细胞形成的细胞生物学机制 近5年来发表论文：

1. Tingting Ren, Jinhan He, Hongfeng Jiang, Luxia Zu, Shenshen Pu, Xiaohui Guo*, and

Guoheng Xu*. Metformin blocks lipolysis in primary rat adipocytes stimulated by tumor

necrosis factor-? or isoproterenol. Journal of Molecular Endocrinology. revised. *Corresponding author.

刘梅芳, 徐国恒*. PAT 家族蛋白在细胞内脂滴代谢过程中的作用. 生理科学进展 2006, 37(2):103-107 *责任作者。

徐冲, 何金汗, 徐国恒*. 脂滴包被蛋白(perilipin)调控脂肪分解. 生理科学进展 2006, 37(3): in press. *责任作者。

Jinhan He, Hongfeng Jiang, John T. Tansey, Chaoshu Tang, Shenshen Pu, and Guoheng Xu*. Calyculin and Okadaic Acid Promote Perilipin Phosphorylation and Increase Lipolysis in Primary Rat Adipocytes. Biochimica et Biophysica Acta -Mol Cell Biol of Lipid. 2006; 1761(2): 247-255. *责任作者。

Xu G*, Sztalryd C, Kimmel AR, and Londos C. Degradation of perilipin is mediated through ubiquitination-proteasome pathway. Biochimica et Biophysica Acta - Mol Cell Biol of Lipid. 2006; 1761(1): 83-90. *责任作者。

Xu G, Sztalryd C, Lu X, Dorward H, Tansey JT, Kimmel AR, and Londos C. Post-translational regulation of adipose differentiation-related protein by the ubiquitin/proteasome pathway. Journal of Biological Chemistry. 2005 Dec; 280(52):42841-7.

Zhang M, Wang X, Zhang D, Xu G, Dong H, Yu Y, Han J. Orphanin FQ antagonizes the inhibition of Ca(2+) currents induced by micro-opioid receptors. Journal of Molecular Neuroscience. 2005;25(1):21-7.

2. 3. 4.

5.

6.

7.

8. Sztalryd C, Xu G, Dorward H, Tansey JT, Contreras JA, Kimmel AR, and Londos C. Perilipin

is Essential for the Translocation of Homone-sensitive Lipase during Lipolytic Activation.

Journal of Cell Biology. 2003 Jun 23; 161(6): 1093-1103. 徐国恒Xu G与Sztalryd C为共同第一作者。

9. Xu G, Rabadan-Diehl C, Nikodemova M, Wynn P, Spiess J, Aguilera G. Inhibition of

corticotropin releasing hormone type-1 receptor translation by an upstream AUG triplet in the 5' untranslated region. Molecular Pharmacology. 2001;59(3):485-492.

朱 毅 教授 简介

朱毅, 教授。 1983年本科毕业于西安医科大学，1986年获医学硕士学位。1993年在瑞士洛桑大学医学院获医学博士学位。1993-1997年先后在美国纽约医学院及美国加州大学Riverside校区（UCR）做博士后训练。1998年1月至2003年9月于UCR生物医学系任研究助理教授。2003年10月起在北京大学医学部生理学与病理生理学系任教授。多年来，一直从事血管内皮激活的信号传导，脂蛋白和血管内皮的相互作用及动脉粥样硬化形成的分子机制等方面研究，共在SCI核心期刊发表论文三十余篇。

他领导的脂代谢与动脉粥样硬化研究室的总体研究方向为以血管内皮激活及动脉粥样硬化形成机制为主的血管生物学。正在进行的课题方向包括： 1， 脂蛋白LDL和HDL与血管内皮的相互作用以及内皮在血管壁脂质平衡中所起的作用

和分子机制。 2， 血流动力学在局灶性动脉粥样硬化形成中的作用机制。 3， 内皮细胞膜亚结构caveolae在血管壁脂质转运中的作用机制及与代谢综合征血管

病变的关系； 4， 不同的代谢性核受体和转录因子之间的相互作用以及代谢综合征、糖尿病发病机制

研究。

5篇代表论文（* 责任作者）：

1. Zhu Y,* Liao HL, Wang N, Yuan Y, Ma K-S, Verna L, Stemerman MB. (2000)

Lipoprotein promotes caveolin-1 and Ras translocation to caveolae: role of cholesterol in endothelial signaling. Arterio Thromb Vasc Biol 20:2465-2470. 2. Zhu Y,* Liao HL, Wang N, Ma K-S, Verna L, Shyy JY-J, Chien S, Stemerman MB. (2001) Low-density lipoprotein-activated p38 in endothelial cells is mediated by Ras. Arterio Thromb Vasc Biol 21:1159-1164. 3. Liao HL, Langmann T, Schmitz G, Zhu Y.* (2002) Native LDL transcriptionaly upregulates ATP-binding cassette transporter-1 in human vascular endothelial cells. Arterio Thromb Vasc Biol 22:127-132. 4. Zhu Y,* Liao HL, Yuan Y, Verna L, Stemerman MB. (2003) Low density lipoprotein

induces eNOS translocation to membrane caveolae via stress fibers. Biochem Biophys Acta 1635：117-126 5. Zhu Y, * Liao H, Xie XF, Yuan Y, Lee T-S, Wang N, Wang X, Shyy JY-J, Stemerman

MB. (2005) Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting LXR. Cardiovas Res 68:425-432. (with editorial 68:353-354)

管又飞教授YOUFEI GUAN, M.D., Ph.D.

Birth date and place: Feb., 15, 1965 Marital status: married, two sons. EDUCATION: M.D. in Medicine, July, 1986

Department of Internal Medicine, Nantong Medical College, Nantong, Jiangsu Province 226001, CHINA

M.S. in Nephrology, Oct., 1989

Department of Internal Medicine, Nantong Medical College, Nantong, Jiangsu Province 226001, CHINA

Ph.D in Nephrology, Nov., 1993

Institute of Nephrology, Beijing Medical University, Beijing 100034, CHINA

Postdoctoral Research Fellow, 1994-1999

Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN37232

APPOINTMENTS: 2005,04-present Changjiang Professor of Pathophysiology The Ministry of Education, People’s Republic of China

2003,09-present Director, Peking University Diabetes Center

2002,09-present Professor of Physiology

Department of Physiology and Pathophysiology, Peking (Beijing) University Health Science Center, Beijing 100083;

1999,09-2002,09 Assistant Professor of Medicine

Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN37232

1989,08-1991,07 Lecturer and Resident Physician

Division of Nephrology, Department of Internal Medicine, The Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu Province 226001, CHINA

1986,09-1989,08 Graduate Research , Teaching Assistant and Resident Physician

Department of Internal Medicine, Nantong Medical College, Nantong, Jiangsu Province 226001, CHINA

MEMBERSHIP OF ACADEMIC ASSOCIATION: Member of American Society of Nephrology (ASN); Member of International Society of Nephrology (ISN); Member of American Physiological Society (APS) Member of Chinese Society of Physiology (CSP) Member of American Diabetes Association (ADA)

Member of American Association for the Advancement of Science (AAAS)

AWARD AND HONOR POSITION:

National Guang Hua Price, 1993, Beijing Medical University

Guest Associate Professor, 1998,05-2001,05, Department of Physiology, Beijing University Medical Center (Beijing Medical University), Beijing100081;

Guest Professor, 2002, 1- present, Division of Nephrology, The General Hospital of PLA, Beijing 100021

ACADEMIC POSITION: Co-Chair, the Symposium “Novel Renal Pathways for Prevention of Diabetes and Obesity-Related Renal and Vascular Disease”. The 2005 Annual Meeting of the ASN, Nov 8-13, Philadelphia, Pennsylvania.

Chairman, the Eicosanoid Receptors Symposium, the International Eicosanoids Conference, Beijing, July 05, 2005

Reviewer, Study Section of Physiology, Pathophysiology and Internal Medicine, National Nature Science Foundation of China (NNSFC), 2004-2008

Reviewer, Study Section of Immunology, National Nature Science Foundation of China (NNSFC), 2005;

Review Committee member, NIDDK RFA-DK-04-012, Study Section of “PKD Research and Translation Core Centers”;

Review Committee member, NIDDK RFA-DK-05-001, Study Section of “Preventing Mitochondrial Oxidative Stress in Diabetes and Obesity”;

Chairman, The Cross-Section Symposium “\2003 FASEB meeting, April 11-14, San Diego.

Chair, The first Diabetes Symposium at Beijing University Medical Center, December 20, 2003;

INVITED REVIEWS: 1. Breyer MD, Zhang Y, Guan Y, Hao CM, Hebert RL, Breyer RM. Regulation of renal

function by prostaglandin E receptors. Kidney Int. 1998; 54 (suppl. 67):S88-S94

2. Guan Y and Breyer MD. Peroxisome proliferator-activated receptors (PPARs): Novel

therapeutic targets in renal disease. Kidney Int. 2001; 60 (1):14-30.

3. Guan Y, Zhang Y and Breyer MD: The role of PPARs in the transcriptional control of

cellular processes. Drug News & Perspective 2002; 15(3):147-154.

4. Guan Y: Peroxisome proliferator-activated receptors (PPARs): Novel therapeutic

targets for prevention of diabetes, dyslipidemia, hypertension and obesity. Minerva Urologica e Nefrologica 2002; 54:65-79

5. Guan Y: PPAR system and its relationship to renal complications of the metabolic

syndrome. J. Am. Soc. Nephrol. (invited review) 2004 Nov;15(11):2801-15.

6. Guan Y: PPAR-gamma agonists and diabetic nephropathy. Current Diabetes Reports

(in presss) (Invited Review).

BOOK CHAPTERS 1. Targeted gene disruption of the prostaglandin E2 EP2 receptor. In Eicosanoids and

Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury. Edited by Honn et al., pp321-326, Kluwer Academic/Plenum Publishers, 2002

2. Vasoactive substances and kidney. in Nephrology . Edited by Haiyan Wang. 2nd ed.

pp 84-180, Public Health Publish House. Beijing. China. 1996

3. Endocrinological Function of Kidney. In Modern Nephrology. Edited by Jije Jiang. 1st

ed. Military Medicine Publish House (in press)

4. Peroxisome proliferator-activated receptors (PPARs) and cardiovascular disease. In

Practical Cardiovascular Receptorology. Edited by Daowen Wang. 1st ed. Science Press, Beijing, ISBN 7-03-009284-8/R.687, 2001, P50-61

5. Scavenger Receptors and cardiovascular disease. In Practical Cardiovascular

Receptorology. Edited by Daowen Wang. 1st ed. Science Press, Beijing, ISBN 7-03-009284-8/R.687, 2001, p62-70

LIST OF PUBLICATIONS 1. Yiang B and Guan Y: Chronic renal failure and recombinant human erythropoietin.

Chinese Journal of Nephrology. 1990. 6:388-391.

2. Guan Y and Qian T: Fibronectin and renal diseases. Advances in Internal Medicine. 1991. 18(5):221-224.

3. Guan Y and Xiu K: Role of reactive oxygen species in gastrointestinal ischemic injury.

Jiangsu Medical Journal 1991. 17(6):322-324.

4. Guan Y, Qian T, and Jiang J: Alteration of the plasma fibronectin concentration in

various kidney disease. Chinese Journal of Nephrology. 1991. 7:81-84.

5. Guan Y, Zhao X, and Jiang J: Significance of hypercoagulable indexes in lupus

nephritis. Tianjin Medical Journal. 1991. 19(6): 349-351.

6. Guan Y, Jiang J, and Qian T: Effect of oral supply of essential amino acids on the

function of kidney and Fc receptor of the peritoneal macrophages in rats with chronic renal failure. Jiangsu Medical Journal. 1991. 17(1):10-12.

7. Zhang, Y, Guan Y, and Jiang J: Structural and functional changes in aged kidneys.

Jiangsu Medical Journal. 1992. 18(1):32-33.

8. Guan Y and Jiang J: Clinical application of recombinant human erythropoietin

(rhuEPO). J. Nephrol. Dialy. Transplant. 1993. 2(1):3-6.

9. Guan Y, Jiang J, Sen Y: Polypeptide growth factors. Advances in Nephrology and Urology. 1993. 13(3):99-103.

10. Guan Y, Wang H: Application of polymerase chain reaction (PCR) technique in

nephrology. Advances in Nephrology and Urology. 1993. 13(4): 162-1655.

11. Guan Y, Jiang J, Qian T: Cyclosporine-associated hypertension. Advances in Internal Medicine. 1993. 20(2):71-73.

12. Guan Y. Decreased expression of c-fos and c-jun oncogenes in aged rat kidneys. Oral

presentation at the First Chinese National Congress of hypertension, fluid, electrolyte metabolism. Apr. 19-21, 1993. Shanghai. China.

13. Guan Y, Xio D and Jiang J: Effect of short-term non-insulin therapy on serum lipid

levels in NIDDM patients. Acta Academia Medicinae Nantong. 1993. 12(4):337-339.

14. Guan Y, Zhang Y, Davis L, Breyer MD: Differential distribution of peroxisome

proliferator-activated receptors (PPARs) along the urinary tract of rabbit and man. Am. J. Physiol. (Renal Physiol.) 1997;273: F1013-F1022.

15. Guan Y, Zhang M, Cho W, Zhang Y, Davis L, Breyer R, Chang, S, DuBois, RN, Hao C,

Breyer MD: Cloning, expression, and regulation of rabbit cyclooxygenase-2 in renal medullary interstitial cells. Am. J. Physiol. (Renal Physiol.) 1997; 273: F18-F26

16. Breyer MD, Zhang Y, Guan Y, Hao CM, Hebert RL, Breyer RM. Regulation of renal

function by prostaglandin E receptors. Kidney Int. 1998; 54 (suppl. 67):S88-S94

17. Guan Y, Zhang Y, Breyer R, Fowler, B, Davis L, Hebert, RL, Breyer MD: PGE2

inhibits renal collecting duct Na+ absorption by activating the EP1 receptor. J. Clin. Invest. 1998;102:194-201

18. Hao, C.-M., Komh?ff M, Guan Y, Redha R, Breyer MD: Selective targeting of

cyclooxygenase-2 reveals its role in renal medullary interstitial cell survival. Am. J. Physiol. 1999; 277:F352-F359

19. Kennedy, CRJ, Zhang Y, Brandon S, Guan Y, Coffee K, Funk CD, Magnuson MA,

Oates JA, Breyer MD, Breyer RM: Hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor. Nature Med. 1999; 5:217-220

20. Guan Y, Zhang Y, Davis L, Redha R, Breyer MD: Expression of peroxisome

proliferator-activated receptor ??in human transitional bladder cancer and its role in inducing cell death. Neoplasia 1999; 1(4):330-339.

21. Zhang Y, Guan Y, Schneider A, Brandon S, Breyer RM, Breyer MD: Characterization

of murine vasopressor and vasodepressor prostaglandin E2 receptors. Hypertension 2000, 35; 1129-1134

22. K?mhoff M, Guan Y, Breyer MD: Enhanced expression of cyclooxygenase-2 in high

grade human transitional cell bladder carcinomas. Am. J. Pathol. 2000, 157:29-35.

23. Guan Y and Breyer MD. Peroxisome proliferator-activated receptors (PPARs): Novel

therapeutic targets in renal disease. Kidney Int. 2001; 60 (1):14-30.

24. Shappell SB, Manning S, Boeglin WE, Guan Y, Wheeler TM, Davis L, Jack G, Breyer

MD, Brash AR: Alterations in lipoxygenase and cyclooxygenase-2 catalytic activity and mRNA expression in prostate carcinoma. Neoplasia 2001; 3(4): 287-303.

25. Guan Y, Zhang Y, Breyer MD: Peroxisome proliferator-activated receptor gamma

(PPAR?) activity is associated with renal microvasculature. Am. J. Physiol. 2001; 281:F1036-F1046.

26. Guan Y, Zhang Y, Schneider A, Davis L, Breyer MD: cDNA cloning, gene mapping,

and intrarenal localization of mouse prostaglandin E synthase. Am. J. Physiol. 2001; 281:F1173-F1177.

27. Zheng F, Fornoni A, Elliot SJ, Guan Y, Breyer MD, Striker LJ, Striker GE. PPAR?

activation suppresses TGF-? mediated Type I collagen expression in mesangial cells. Am. J. Physiol. (Renal Physiol.) 2002; 282:F639-648

28. Guan Y and Breyer MD: Reply from the authors “Fibrates-induced renal injury”.

Kidney Int. 2002; 61(1):354-355.

29. Jones JR, Shelton KD, Guan Y, Breyer MD, Magnuson MA. Generation and

functional confirmation of a conditional null PPAR? allele in mice. Genesis 2002;

32:134-137.

30. Guan Y, Zhang Y and Breyer MD: The role of PPARs in the transcriptional control of

cellular processes. Drug News & Perspective 2002; 15(3):147-154, Review

31. Panzer U., Scneider A, Guan Y, Reinking R, Zahner G, Harendza S, Wolf G, Thaiss F,

Stahl RAK: Activation of the peroxisome proliferator-activated receptor ? (PPAR?) increases MCP-1 expression and monocyte infiltration in experimental glomerulonephritis. Kidney Int. 2002 Aug;62(2):455-64

32. Guan Y, Stillman B, Breyer R, Zhang Y, Davis L, Redha R, Nian C, Breyer MD:

Cloning and functional expression of the rabbit prostaglandin EP2 receptor. BMC Pharmacology 2002, 2:14 (27 June 2002)

33. Schneider A, Zhang Y, Guan Y, Davis L, Breyer RM, Breyer MD: Differential,

inducible gene targeting in renal epithelia, vascular endothelium, and viscera of Mx1Cre mice. Am. J. Physiol. (Renal Physiol.) 2003;284:F411-F417.

34. Saito S, Guan Y, Davis L, Breyer M: Expression of the prostaglandin F receptor (FP)

gene along the mouse genitourinary tract. Am. J. Physiol. (Renal Physiol.) 2003; 284:F1164-1170.

35. Peti-Peterdi J, Komlosi P, Bebok Z, Guan Y, Schneider A, Qi Z, Redha R, Breyer M,

Bell PD. Luminal NaCl delivery regulates basolateral PGE2 release from intact Macula densa cells. J. Clin. Invest. 2003 Jul;112(1):76-82

36. Zhang Y, Schneider A, Reena Rao, Wendell J Lu, Fan XF, Davis L, Breyer R, Beryer

MD and Guan Y: Genomic structure and genitourinary expression of mouse cytosolic prosaglandin E2 synthase (cPGES) gene. Biochimica et Biophysica Acta- Mol. Biol. Lipid 2003: 1634; 15-23.

37. Hebert RL, Guan Y, Fowler B, Breyer MD: PGE2 inhibits renal collecting duct

sodium transport and the release of cell calcium by activating the EP1 receptor subtype. Recent Res. Devel. Physiol. 2003; 1:307-317.

38. Schneider A, Zhang Y, Zhang M, Lu W, Rao R, Fan X, Redha R, Davis L, Breyer RM,

Harris R, Breyer MD, Guan Y: Membrane-associated PGES-1 (mPGES-1) is co-expressed with both COX-1 and COX-2 in the kidney. Kidney Int. 2004; 65:1205-1213.

39. Ma L, Ma S-L, Kanjanabuch T, Guan Y, Zhang Y, Brown NJ, Swift L.L., Mcguinness

O.P, Wasserman D.H., Fogo A. B. Prevention of obesity and insulin resistance in mice lacking PAI-I gene. Diabetes 2004; 53:336-346.

40. Schneider A, Guan Y, Zhang Y, Magnuson MA, Loftin CD, Langenbach R, Breyer

RM, and Breyer MD: Generation of mice suitable for conditional prostaglandin EP4 receptor deletion. Genesis 2004; 40:7-14

41. Guan Y. Invited Review: PPAR family and renal complications of the metabolic

syndrome. J. Am. Soc. Nephrol. 2004 Nov;15(11):2801-15

42. Zheng F, Chen Q, Plati A-R, Ye S-Q, Berho M, Banerjee A, Potier M, Jaimes EA, Guan

Y, Hao C, Striker LJ, Striker GE. The glomerulosclerosis of aging: Contribution of the proinflammatory mesangial cell phenotype to macrophage infiltration. Am. J. Pathol. 2004; 165:1789-1798;

43. Wu J, Zhang Y, Yang G, Wang N, Zhu Y, Wang X, Davis L, Breyer MD, Guan Y:

Liver X receptor alpha mediates cholesterol efflux in cultured mesangial cells. . Am. J. Physiol. (Renal Physiol.) 2004; 287:F886-F895;

44. Xiong Z, Huang H, LI J, Guan Y, Wang H: Anti-inflammatory effect of PPARg in

cultured human mesangial cells. Renal Failure 2004; 26:497-505;

45. Zeng X-K, Guan Y, Remick D. G. Wang X. Signal pathways underlying

homocysteine-induced production of MCP-1 and IL-8 in cultured human whole blood. Acta Phamacologica Sinica 2005; 26(1):85-91;

46. Wang G, Wei J, Guan Y, Jin N, Mao J, Wang X: PPAR? agonist rosiglitazone reduces

inflammatory response in type II diabetic patients with coronary arterial disease flowing coronary angioplasty. Metabolism 2005; 54(5):590-597.

47. Yang F, Zhang Z, Xin D, Shi C, Wu JP, Guo YL, Guan Y: PPAR? ligands induce cell

cycle arrest and apoptosis in human renal carcinoma cell Lines. Acta Pharmacologica Sinica 2005;26(6):736-761.

48. Guan Y, Hao C, Rao R, Cha DR, Kohan D, Magnuson M, Zhang Y, Breyer MD:

PPAR? regulates systemic fluid volume by activating sodium absorption in the collecting duct._ Nature Medicine 2005 Aug;11(8):861-866;

49. Hebert R, Saito O, Carmosino M, Yang G, Jackson C, Qi Z, Zhang Y, Guan Y, Breyer

M: Characterization of the rabbit PGF2alpha receptor (FP) and its Gi restricted signaling pathway inhibiting water absorption in renal collecting duct. J. Biol. Chem. 280(41):35028-37;

50. Zhan P, Alander C, Kaneko H, Pilbeam CC, Guan Y, Zhang Y, Breyer MD, Raisz LG:

Effect of deletion of the prostaglandin EP4 receptor on stimulation of calcium release from cultured mouse calvariae: Impaired responsiveness in heterozygous. Prostaglandins Other Lipid Mediat. 2005 Dec;78(1-4):19-26.;

51. Xie L, Chang L, Guan Y, Wang X: C-reactive protein augments interleukin-8

secretion in human peripheral blood monocytes. J Cardiovasc Pharmacol. 2005 Nov;46(5):690-6.

52. Zhang Y & Guan Y: PPAR-gamma agonists and diabetic nephropathy. Current Diabetes Reports 2005；5：470-475 (Invited Review).

53. Park WC, Zhang Y, Wu J, Lu W, Fan X, Davis L, Pozzi A, Capdevila J, Zeng F, Breyer

M, Guan Y: PPARalpha agonist improves diabetic parameters and diabetic nephropathy in db/db mice. Kidney Int. 2006 (in press).

54. Zhang Y, Wu J, Yang G, Lu W, Hwang M-T, Davis L, Breyer M, Guan Y: Liver X

receptor (LXR) agonist TO901317 upregulates SCD1 expression in renal proximal straight tubule. Am J Physiol (Renal Physiol) (in press);

55. Park CW, Kim HW, Chung HW, Lim SW, Yang CW, Chang YS, Sugawara A, Guan Y,

Breyer MD: Accelerated Diabetic Nephropathy in Mice Lacking the Peroxisome

Proliferator-Activated Receptor-?. Diabetes (in revision);

56. Nakagawa K, Holla VR, Wei Y, Wang W-H, Wei S, Mei S, Cha DR, Price E Jr, Zent

R, Pozzi A, Breyer MD, Guan Y, Falck JR, Waterman M, Capdevila JH: Salt sensitive hypertension is associated with cytochrome P450 epoxygenase-mediated changes in the activity of the renal epithelial sodium channel. Nature (in revision);

57. Guan Y, Zhang Y, Qi Z, Yang G, Davis L, Wei MF, Fan XF, Breyer R, Breyer MD:

Prostaglandin E2 receptor EP1 is an vasopressor in blood pressure regulation. Cell Metabolism (submitted);

58. Cha DR, Su D., Zhang Y, Fan X, Breyer M, Guan Y: Peroxisome Proliferator

Activated Receptor ? Deficiency Protect From High Fat Diet-Induced Insulin Resistance in Aging Mice. Diabetologia (submitted);

59. Cha DR, Su DM, Han JY, Zhang Y, Fan XF, Breyer MD, Guan Y: PPAR ??? Dual

Agonist Tesaglitazar (AZ242) attenuates Diabetic Nephropathy in db/db Mice. Diabetes (submitted);

60. Zhang Y, Lu W, Fan XF, Shappell SB, Roberts R, Davis L, Guan Y: PPAR? agonists

decrease albuminuria and slow progression of diabetic nephropathy in obese diabetic db/db mice. (in preparation).

61. Zhang Y, Fan XF, Capdevila J, Breyer MD, Guan Y: Activation of a cytochrome p450

arachidonate epoxygenase contributes to normalization of blood pressure produced by the antidiabetic PPAR? ligand troglitazone in SHR rats. (in preparation).

汪南平 教授 博士生导师

专业：生理学

最后学历：医学博士

研究专长：心血管分子生物学与生物工程学 工作单位：北京大学心血管研究所

北京大学医学部生理学与病理生理学系（兼）

联系地址：北京市海淀区学院路38号 北京大学医学部心血管研究所

血管分子生物学与生物工程学研究室 电话：10-82801146

E-mail:npwang@bjmu.edu.cn 学习和工作经历

1983-1986西安医科大学 医学硕士

1986-1988西安医科大学第二临床学院 医师 1989-1990日本北里大学医学部 访问学者 1991-1994北京医科大学 医学博士

1994-1995德国柏林自由大学医学中心 访问学者

1995-1996美国印地安那大学医学院微生物与免疫学系 博士后 1996-2000美国加利福尼亚大学（河滨）生物医学系 博士后

2000-2002美国加利福尼亚大学（河滨）生物医学系 研究助理教授 2003-2005美国加利福尼亚大学（圣地亚哥）生物工程系 研究助理教授 2003- 北京大学医学部心血管研究所 教授 副所长

研究方向代谢性核受体

基因转录调控与功能基因组学 机械-生物信号传导 承担课题

American Heart Association Scientist Development Grant 教育部985专项基金 国家自然科学基金 教育部创新团队基金 主要论文

Eberle,J.; Garbe,C.; Wang,N.; Orfanos,C.E. Incomplete expression of the tyrosinase gene family (tyrosinase, TRP-1, and TRP-2) in human malignant melanoma cells in vitro. Pigment Cell Res. 1995; 8:307-313

Farthmann,B.; Eberle,J.; Krasagakis,K.; Gstottner,M.; Wang,N.; Bisson,S.; Orfanos,C.E. RT-PCR for tyrosinase-mRNA-positive cells in peripheral blood: evaluation strategy and correlation with known prognostic markers in 123 melanoma patients. J.Invest Dermatol. 1998; 110:263-267

Wang, N., Verna, L., Hardy, S., Forsayeth, J., Zhu, Y., Ma, K.S., Stemerman, M.B. Adenovirus-mediated overexpression of c-Jun and c-Fos induces intercellular adhesion

molecule-1 and monocyte chemo-attractant protein-1 in human endothelial cells. Arterioscler Thromb Vasc Biol. 1999; 19:2078-2084

Zhu, Y., Liao, H.L., Wang, N., Friedli, O., Verna, L., Stermerman, M.B. Low-density

lipoprotein activates Jun N-terminal kinase (JNK) in human endothelial cells. Biochimica et Biophisica Acta 1999; 1436:557-64

Wang, N., Verna, L., Hardy, S., Ma, K., Zhu, Y., Birrer, MJ., Stemerman, M.B. c-Jun triggers apoptosis in human vascular endothelial cells. Circ Res. 1999; 85: 387-393

Liao, H.L., Zhu, Y., Wang, N., Verna, L., Stemerman, M.B. Selective activation of endothelial cells by the antioxidant pyrrolidine dithiocarbamate: involvement of c-Jun and Jun N-terminal kinase activation. Endothelium 2000; 7:121-133

Zhu, Y., Liao, H., Wang, N., Yuan, Y., Ma, K., Verna, L., Stemerman, MB. Lipoprotein promotes caveolin-1 and ras translocation to caveolae. Role of cholesterol in endothelial signaling. Arterioscler Thromb Vasc Biol. 2000; 20:2465-2470

Zhu, Y., Liao, H., Wang, N., Ma, K., Verna, L., Shyy, J., Chien, S., Stemerman, MB.

Low-density lipoprotein-activated p38 is mediated by ras. Arterioscler Thromb Vasc Biol. 2001; 21:1159

Wang, N., Verna, L., Zhu, Y., Liao, H., Ballard, A., Stemerman, MB. Adenovirus-mediated overexpression of a dominant negative mutant of c-Jun prevents intercellular adhesion

molecule-1 induction by LDL-A critical role for AP-1 in endothelial activation. Arterioscler Thromb Vasc Biol. 2001; 21: 1414-1420

Yuan, Y., Verna, L., Wang, N., Liao, H., Ma, K., Wang, Y., Zhu, Y., Stemerman, MB. Cholesterol enrichment induces intercellular adhesion molecule-1 in human vascular endothelial cells. Biochimica et Biophisica Acta. 2001; 1534: 139-48

Wang, N., Stemerman, MB. Ref-1 and transcriptional control of endothelial apoptosis. Circ Res 2001; 88:1223 –1225（Editorial）

Wang, N., Verna, L., Chen, N., Chen, J., Li, H, Forman, B.M., Stemerman, MB. Constitutive activation of PPAR-suppresses expression of adhesion molecules in vascular endothelial cells. J Biol Chem 2002; 277: 34176-34181

Wang, N. Cell Adhesion Molecules (CAMs). Encyclopedia of the Neurological Sciences, (Aminoff, MJ., and Daroff, RB, ed) Vol. 1, pp 541-542, Academic Press, San Diego, 2003

Wu J, Zhang Y, Wang N, Davis L, Yang G, Wang X, Zhu Y, Breyer MD, Guan Y. Liver X receptor alpha (LXRalpha) mediates cholesterol efflux in glomerular mesangial cells. Am J Physiol Renal Physiol. 2004; 287:F886-895

Liu,Y., Zhu, Y., Rannou, F., Lee, T-S., Formentin, K., Zeng, L., Yuan, X., Wang, N., Chien, S., Forman, B.,Shyy, Y-J. Laminar flow activates peroxisome proliferator-activated receptor-in vascular endothelial cells. Circulation 2004; 28-1133

Yan H, Guo Y, Zhang P, Zu L, Dong X, Chen L, Tian J, Fan X, Wang N, Wu X,Gao W. Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors. Biochem Biophys Res Commun. 2005; 26:336:287-298

Zhu Y, Liao H, Xie X, Yuan Y, Lee TS, Wang N, Wang X, Shyy JY, Stemerman MB. Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting liver X receptor (LXR). Cardiovasc Res. 2005; 68:425-32

张鹏, 汪南平. 脂肪因子与代谢综合征.基础医学与临床, 2006, 26:20-26.

Wu, C, Li ,Y ,. Haga, J, Wang,N, Lian, I, Su, F, Usami, S and S Chien. Roles of MAP kinases in the regulation of bone matrix gene expressions in human osteoblasts by oscillatory fluid flow. J Cell Biochem. 2006 Jan 26; [Epub ahead of print]

Wang,N.; Miao,H.; Li,Y.S.; Zhang,P.; Haga,J.H.; Hu,Y.; Young,A.; Yuan,S.; Nguyen,P.; Wu,C.C.; Chien,S. Shear stress regulation of Kruppel-like factor 2 expression is flow pattern-specific. Biochem Biophys Res Commun. 2006; 341:1244-1251

Wu,X.; Ma,J.; Han,J.D.; Wang,N.; Chen,Y.G. Distinct regulation of gene expression in human endothelial cells by TGF-beta and its receptors. Microvasc.Res.2006; 71:12-9

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