油橄榄叶中总黄酮的提取工艺优化

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油橄榄叶中总黄酮的提取工艺优化

作者:罗茜

来源:《湖北农业科学》2014年第06期

摘要:以油橄榄(Olea europaea L.)叶片为原料,以芦丁为对照品,以乙醇体积分数、提取温度、料液比、回流时间为考察因素,以总黄酮提取率为指标,先通过单因素试验,再采用正交试验设计优选提取工艺。结果表明,最佳提取工艺为乙醇体积分数90 %、提取温度60 ℃、料液比1∶19、回流时间1.5 h,在此条件下,总黄酮的提取率为2.280%。该提取工艺合理、简单,可用于大规模生产。

关键词:正交设计;油橄榄(Olea europaea L.)叶;总黄酮

中图分类号:S667.5;TS201.2 文献标识码:A 文章编号:0439-8114(2014)06-1406-03 Optimization of Extracting Total Flavonoids from Olive Leaf LUO Qian

(School of Applied and Chemical Engineering, Xichang College,Xichang 615013, Sichuan,China)

Abstract: Using olive leaf as materials, rutin as control, and ethanol concentration, extraction temperature, material to solvent, refluxing extraction time as evaluation factors and the extraction rate of the total flavonoids as an indicator, the technology extracting the total flavonoids from olive leaf was optimized with single-factor experiment and orthogonal design. The results showed that the optimal technology were ethanol concentration 90%, extraction temperature 60 ℃, material to solvent 1∶19, refluxing extraction time 1.5 h. The extraction ratio of flavonoids was 2.2815% under the optimized conditions. The extraction method was reasonable and simple, and could be used for industrial production.

Key words: orthogonal experiment; olive(Olea europaea L.) leaf; total flavonoids 油橄榄(Olea europaea L.)为木犀科木樨榄属植物,常绿阔叶乔木,又名洋橄榄、齐敦果[1]。最近几年,随着人们物质生活水平的提高,对营养保健的要求也逐年提高。油橄榄果虽可加工成素有“植物油皇后”之称的橄榄油,但是我国的橄榄油产量并不大,目前主要依靠进口。国内外对于橄榄油的报道较多,但是油橄榄的果渣、废水和橄榄叶还鲜有研究报道。 国外的油橄榄叶提取物已经广泛应用于化妆品、食品和药品[2]。油橄榄叶中活性成分主要有有机酸、蛋白质、氨基酸、黄酮等[3],即说明可大力发展利用油橄榄叶片。

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